Reduce costs, reduce animal use, and reduce time spent on no-go compounds with the ABST system, a collaboration between DSI and BASi®.
Case Study: Novel Safety Pharmacology Multiparameter Model
This collaboration makes it possible to collect multiple streams of data simultaneously from individual animals. The ability to collect blood at the same time as other safety data eliminates the use of satellite groups. Subjects can be re-used after a washout period, leading to further reduction in the use of animals. Since the system is fully automated, it is possible to reduce staff hours compared to traditional methods. Additional money savings arise thanks to better data. The ABST makes it possible to make earlier go/no-go decisions so money isn’t wasted on compounds that will not make it to market.
The ABST offers full automation for dosing, blood sampling, and the collection of safety data. This allows technicians to use time effectively. For example, an ABST experiment can commence in the morning and run unattended throughout the day, freeing up time for additional work. This can make your staff more effective than ever. Elimination of satellite groups further reduces the amount of time spent on each compound. Finally, early decisions can be made with high quality data, reducing the overall time spent on non-viable compounds.
With satellite groups it is difficult to ensure that all study parameters are the same. In fact, manual pharmacokinetic sampling is often very stressful for research animals, while great care is taken to minimize human intervention during the collection of safety data. The ability to eliminate satellite groups with ABST allows for better control over study variables. Data collection is further refined by achieving a within-subject experimental design and capturing inter-animal variability. Additionally, the ABST makes it possible to compare information across organ systems to determine time and exposure-dependent effects. All of these features make it possible to form early and educated decisions during drug development.
Culex® has the flexibility to collect multiple streams of data from a variety of subjects. By utilizing a unique movement responsive caging system called the Raturn, it is possible to replace the liquid swivel. This permits experimenters to connect multiple inlet and outlet lines to the subject for programmed automated dosing and sampling of blood, urine, feces, bile, microdialysates and more.
DSI telemetry systems provide a reliable, cost-effective means of physiological monitoring in subjects of any size. To integrate this system with BASi Culex ®, a specialized antenna was developed. This antenna permits clean data collection with minimal noise. The antenna is placed around the cage and does not interfere with its rotation. DSI’s antenna and receiver make it possible to collect heart rate, BP/LVP, temperature and biopotential lead data simultaneous with the biofluid collection from the BASi Culex ®.
This combination gives researchers a powerful tool for performing integrative pharmacology studies. BASi® and DSI are proud to be leading the way into a future that uses fewer animals and resources to achieve better data.