Open Flow Microperfusion (OFM)

Interstitial Sampling of Large and Lipophilic Molecules Using Membrane-Free Probes

Products and Contract Research Services

‚ÄčOpen flow microperfusion (OFM) is a novel, tissue-specific sampling technique that builds on the strengths of minimally invasive, interstitial sampling methods such as microdialysis (MD). OFM uses patented membrane-free probes with macroscopic openings which create an interface to the surrounding interstitial fluid (ISF). OFM samples represent a diluted, but unfiltered ISF and thus contain all substances regardless of their physico-chemical properties (e.g. lipophilicity) or molecular size (e.g. peptides, antibodies, nanocarriers). Continuous OFM sampling provides dynamic substance profiles and absolute substance quantification in the investigated target tissues and can currently be performed in brain (cOFM) and skin/adipose tissue (a/d OFM).

 

OFM is a valuable tool in neuroscience, dermatology, oncology, biomarker research and PK-PD studies.

 

Minimally invasive open flow microperfusion (OFM) can be used …

  • to continuously sample ISF without the limitations of membranes.
  • for direct access to the interstitial fluid (ISF) of brain (cOFM), skin (dOFM), and adipose tissue (aOFM).
  • for multiple sampling sessions in the same animal, thereby reducing the number of animals.

OFM derives better data and drives better decisions

  • Monitoring of substances with excellent temporal resolution.
  • No limitation regarding lipophilicity, protein binding, molecular size including antibodies, nanocarriers up to living cells.
  • Absolute quantification of substances.

Competitive edge in characterizing:

  • Neurotransmitters
  • Bound and Unbound Drug
  • Peptides and Proteins
  • Antibodies
  • Nanoparticles/Nanocarriers
  • Enzymes
  • Vesicles
  • Cells
    • In mice, rats, dogs, pigs, primates

OFM reduces costs and development time by providing the pharmacological profile of a drug at an earlier phase in drug development.

Resources

BASi can assist you with setting up your own OFM platform in your lab. Contact us with your request.

Contact us if you are interested in outsourcing a preclinical OFM study with Joanneum Research.

Click to download cOFM flyer.

Click for published literature on cOFM.

 Click for published literature on a/d OFM

Click to view cOFM video.  

Read OFM Press Release.